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. 2019 Dec 19;21(1):37. doi: 10.3390/ijms21010037

Figure 3.

Figure 3

Full-length dysferlin-HA restores G-actin incorporation in dysferlinopathy myoblasts. Control C25 or dysferlinopathy DYSF3 myoblasts were transfected with full-length dysferlin-HA (FL-dysferlin-HA), and 24 h fluorescently tagged G-actin incorporation was assayed as previously described. FL-dysferlin-HA expression was assayed by immunofluorescence using a monoclonal antibody against dysferlin and a Cy3-conjugated anti-rabbit secondary antibody. Confocal images were acquired at the equatorial plane of the cells using identical exposure settings between compared samples. (a) C25 and DYSF3 myoblasts with fluorescent actin filaments (green) and FL-dysferlin-HA immunostaining (red). Scale bar = 10 µm. Insets show digital magnification of the boxed areas. (b) Bars represent means ± SEM. Actin fluorescence intensity was measured in a single focal plane at the equator of cells and normalized by the cell area. The number of cells analyzed from four different cultures is indicated in parentheses. No significant differences (NS) were found (t-test).