Figure 2.

Induction of RON by lysophosphatidic acid (LPA) in T24 bladder cancer cells. (A,B) RT-PCR and Western blot analyses of the effect of LPA on RON mRNA and protein expression in T24 cells, respectively. Cells were incubated with 5 μM LPA for the indicated durations. (C) T24 cells were transiently transfected with the pGL3–RON reporter construct overnight. The transfected cells were incubated with 5 μM LPA for 0–8 h, and luciferase activity was measured with a luminometer. Bars show the mean standard deviation from three measurements.