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. 2019 Dec 21;21(1):98. doi: 10.3390/ijms21010098

Figure 1.

Figure 1

Construction and validation of mutant ∆preA and complemented strain C-preA of Aeromonas veronii. (a) PCR detection of deletion strain ∆preA (M: 5000 bp marker; 1, 2: the suspected mutant strain; 3: the wild-type strain of TH0426); (b) PCR verification of complemented strain C-preA (M: 2000 bp marker; 1: the wild-type strain; 2: C-preA); 2: C-preA; (c) analysis of the relative mRNA expression of the preA gene; (d) PCR analysis of the genetic stability of partial deletion strain (M: 5000 bp marker; 1–21: the mutant strain ∆preA; 22–23: the wild-type TH0426; 24: negative control); (e) genetic stability analysis of partial complemented strain C-preA (M: 2000 bp marker; 1–8: the complemented strain C-preA; 9–10: the parent strain).