Table 3.
Bacterial strains and plasmids used in this study. Ampicillin (Amp), chloramphenicol (Cm).
| Strains or Plasmids | Description | Source or References |
|---|---|---|
| Strains | ||
| A. veronii TH0426 | Wild-type strain, Ampr | This study |
| ∆preA | preA deletion mutant of TH0426, Ampr | This study |
| C-preA | ∆preA complemented with the whole preA gene | This study |
| E. coli Trans1-T1 | F- φ80(lacZ)ΔM15ΔlacX74hsdR (rk−, mk+) ΔrecA1398endA1tonA | TransGene Biotech |
| E. coli DH5α-λpir | λpir lysogen of DH5α | Stored in our lab |
| E. coli WM3064 | thrB1004 pro thi rpsL hsdS lacZ ∆ M15RP4-1360(araBAD)567 ∆ dapA1341: [erm pir(wt)] | Stored in our lab |
| Plasmids | ||
| pEASY-Blunt Zero | TA cloning vector, Ampr | TransGene Biotech |
| pEASY-UD preA | Carrying a 1015 bp fragment upstream and a downstream 979 bp fragment of the preA ORF, Ampr | This study |
| pRE112 | pGP704 suicide plasmid, pir depengent, oriT, oriV, sacB, Cmr | Stored in our lab |
| pRE112-UDpreA | pRE112 carrying a 1015 bp fragment upstream and a downstream 979 bp fragment of the preA ORF, Cmr | This study |
| pBBR1-MCS | Broad-host range vector, Cmr | Stored in our lab |
| pBBR-preA | pBBR carrying a fragment of 847 bp containing the promoter and preA ORF, Cmr | This study |