Table A1.
Primers for construction of ∆preA and C-preA.
| Target Gene | Primer | Sequence (5′-3′) |
|---|---|---|
| preA upstream region | P1-1 (Xba I) | CGTCTAGAGCCGAGGTGACCGAGGCGACCATGG |
| P1-2 | GGAACTTCTGGCTGCTTCTTTGTACTGATTT | |
| preA downstream region | P1-3 | AAGAAGCAGCCAGAAGTTCCGCGCCCATT |
| P1-4 (Sac I) | TAGAGCTCCCGAGAGCTTGAGGGTGCCGT | |
| preA region outside | P3-1 | GCGCCATGGACGAAGTTGACC |
| P3-2 | ATCCGCAGCGCCAGATCGAA | |
| preA ORF internal sequence | P4-1 | AGGCCACACCCACCACAGGCT |
| P4-2 | CTGCAAACCCTGGCGGCTCC | |
| preA promoter sequence | P5-1 (Pst I) | AACTGCAGTTTCCATCCTCAGTAAATAATGA |
| P5-2 | TCAGTACAAACCGTTCGTTCCTTCATCTT | |
| preA ORF sequence | P6-1 | GAACGAACGGATGGGCGCGGAACTTCTGC |
| P6-2 (Hind III) | GCAAGCTTTCAGTACAAAGAAGCAGCAGGCC | |
| 16S rRNA sequence (used for qRT-PCR) |
P7-1 | GCCACGTCTCAAGGACACAG |
| P7-2 | TGGGGAGCAAACAGGATTAGA | |
|
preA ORF sequence (used for qRT-PCR) |
P8-1 | TCTATGGCATACCGGAAAAGG |
| P8-2 | ACTGAACACCGCAAAGAGCA | |
| pRE112 vector | P9-1 | GCGATGAGTGGCAGGGC |
| P9-2 | TTACCGACTGCGGCCTGAGT | |
| pBBR1-MCS vector | P10-1 | TAAGTTGGGTAACGCCAGG |
| P10-2 | GAGTTAGCTCACTCATTAGGC |