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. 2019 Dec 25;21(1):172. doi: 10.3390/ijms21010172

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Diosgenin activates checkpoint proteins. (A) Incubation of MCF-7 (left panel) and Hs578T (right panel) cells with diosgenin increases expression of checkpoint kinase 1 (Chk1) and phosphorylated (activated) forms of Chk1 (p-Chk1^Ser345), p-p53^Ser15, p21, and p-Cdc25C^Ser216. Densitometric analyses for (B) MCF-7 and (C) Hs578T cells were performed using Digital Protein DNA Imagineware. β-actin was used to normalize the amount of protein in each lane. Data are shown as means ± SD for three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control (Ctrl, 0 µM) group.

Diosgenin activates checkpoint proteins. (A) Incubation of MCF-7 (left panel) and Hs578T (right panel) cells with diosgenin increases expression of checkpoint kinase 1 (Chk1) and phosphorylated (activated) forms of Chk1 (p-Chk1^Ser345), p-p53^Ser15, p21, and p-Cdc25C^Ser216. Densitometric analyses for (B) MCF-7 and (C) Hs578T cells were performed using Digital Protein DNA Imagineware. β-actin was used to normalize the amount of protein in each lane. Data are shown as means ± SD for three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control (Ctrl, 0 µM) group.