Table 1.
Tyrp1tr parameters determined from the gel-filtration chromatography and dynamic light scattering.
| pH 7.2 | ||||||
| Temperature (°C) | GF | DLS | ||||
| Molecular Weight (kDa) | Area of Peak (%) | Hydrodynamic Diameter (nm) | ||||
| Peak A | Peak B | Peak A | Peak B | Peak A | Peak B | |
| 4 | 57.6 ± 1.9 | - | 98.2 ± 0.2 | - | 7.8 ± 0.1 | - |
| 25 | 59.6 ± 1.0 | - | 96.4 ± 1.2 | - | 8.5 ± 0.1 | - |
| 31 | 58.6 ± 1.4 | 961.3 ± 155.8 | 87.3 ± 1.0 | 8.7 ± 0.4 | 9.8 ± 0.8 | - |
| 37 | 56.5 ± 1.4 | 1074.8 ± 253.4 | 36.9 ± 0.3 | 48.7 ± 0.3 | 13.8 ± 0.5 | 52.8 ± 0.4 |
| 43 | 55.0 ± 1.8 | 1469.3 ± 500.1 | 7.6 ± 0.2 | 74.3 ± 1.8 | 30.4 ± 4.5 | 71.5 ± 1.3 |
| pH 5.5 | ||||||
| Temperature (°C) | GF | DLS | ||||
| Molecular weight (kDa) | Area of peak (%) | Hydrodynamic diameter (nm) | ||||
| Peak A | Peak B | Peak A | Peak B | Peak A | Peak B | |
| 4 | 54.3 ± 0.9 | - | 93.6 ± 0.5 | - | 7.9 ± 0.6 | - |
| 25 | 55.0 ± 0.9 | - | 95.1 ± 0.6 | - | 7.6 ± 1.5 | - |
| 31 | 54.0 ± 0.5 | 1011.7 ± 16.5 | 88.8 ± 8.1 | 8.0 ± 8.8 | 16.1 ± 0.9 | - |
| 37 | 56.9 ± 0.9 | 989.1 ± 48.3 | 71.1 ± 3.2 | 13.8 ± 10.6 | 19.0 ± 0.6 | - |
| 43 | 57.6 ± 1.9 | 1023.6 ± 33.3 | 18.7 ± 3.1 | 17.9 ± 15.8 | - | - |
To estimate the molecular weight of Tyrp1tr (kDa), Superdex 200 Increase 10/300 column was pre-calibrated with the GF standards (Bio-Rad): thyroglobulin (670 kDa), γ-globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), and vitamin B12 (1.3 kDa). The Area of peaks (%) was measured using the UNICORN 7.0 software (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). The hydrodynamic diameter was calculated from DLS using the Kalliope Professional 2.2.3 software (Anton Paar, Graz, Austria).