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. 2019 Dec 18;21(1):8. doi: 10.3390/ijms21010008

Figure 1.

Figure 1

Evidence for a new exon of Nephrocan in mouse. (a) The sequence of Nepn gene in GenBank runs from 52,388,864 bp to 52,404,613 bp on chromosome 10 (top). Schematic of transcripts reported on Ensemble database and primer designing for RT-PCR (bottom). (b) Mouse kidney tissue was used to study the expression of the novel transcript by RT-PCR. NC, negative control with no DNA template. (c) Schematic representation of the genomic organization of mouse Nepn leading to alternative splicing. Rectangular boxes refer to exons (size of boxes are indicative of the relative size of exons) and interconnecting lines as introns. The dashed puckered lines show the splicing pattern of the exons. The novel transcript, named Nepn isoform a, is formed by splicing of Exon1a with Exon2 skipping Exon1b. The translation initiation site “AUG” is indicated with a downward arrow in the new transcript. (d) Alignment of Nepn isoform b (upper sequence) and isoform a (lower sequence) obtained using the Sequence Manipulation Suite programs. Full blot is showed in Supplementary Information.