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Measurement of reactive oxygen species (ROS) and cell death in wheat cultivar CM42 inoculated with CYR32 and V26. Histochemical analysis for superoxide anion radicals (O2˙ˉ) and hydrogen peroxide (H2O2) by nitro blue tetrazolium (NBT) (A) and 3,3-diaminobenzidine (DAB) (B) staining, respectively. (C) Degree of cell death was visually detected by trypan blue staining. CK, un-inoculated wheat plants. 24–120 hpi represent 24, 48, and 120 h post-inoculation.
