Figure 2.
The anti-leukemic effects of Aca are mediated by caspase independent mechanisms (A) OCI-M2S and OCI-M2R cells (0.5 × 106 cells/mL) were treated with increasing concentrations of Aca for 48 h at 37 °C, in the presence or in the absence of 20 µM Z-VAD-fmk, a pan-caspase inhibitor. Cell metabolism was measured using the XTT assay as described in Materials and Methods section. (B) OCI-M2S and OCI-M2R cells (0.5 × 106 cells/mL) were treated with 1 µM of Aza for 48 h at 37°C, in the presence or in the absence of 20 µM Z-VAD-fmk, a pan-caspase inhibitor. Cell metabolism was measured using the XTT assay as described in Materials and Methods section. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns: not significant.