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. 2019 Dec 30;21(1):256. doi: 10.3390/ijms21010256

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence staining. Expression of Ki67 (green, stained cells are indicated with arrows), involucrin (green) and loricrin (green) monitored in healthy (H) or psoriatic (P) skin substitutes treated or not with methotrexate (MTX), balsacone A (BA), balsacone B (BB), balsacone C (BC), (±)-balsacone K (BK), and (±)-iryantherin-D (BD). Nuclei were stained with Hoechst (blue). Three substitutes of each condition were analyzed and the results were confirmed with two independent experiments. (Scale bar = 100 μm).