Figure 6.

Association between protein Amuc_1434* and Muc2. (A) Determined Muc2 expression in LS174T and HeLa cells using Western blot. (B) Effect of Amuc_1434* concentration in the range of 0–16 μg/mL on adhesion of LS174T cells. HeLa cells were used as negative control. LS174T and HeLa cells bound to the wells were photographed. Crystal violet was extracted, and the remaining adherent cells were quantified by spectrophotometric absorbance at 570 nm. Scale bar = 50 μm (n = 3, *: p < 0.05; **: p < 0.01). The black line indicated which two groups were compared for significant differences in the column chart.