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. 2019 Dec 27;21(1):201. doi: 10.3390/ijms21010201

Figure 2.

Figure 2

Upregulation of miR-195 post MI. This miRNA was upregulated in fibroblasts (A) as well as in exosomes obtained from cardiomyocytes (cardiosomes, B) isolated seven days after MI (n ≥ 6 mice/group); Smad7 mRNA was significantly down-regulated whereas αSMA and periostin, known markers of myofibroblast activation, were upregulated in fibroblasts post-MI (A). In panel C, cardiosome preparations were re-suspended in 300 mL PBS and spiked with 20 ρmol of a synthetic oligonucleotide corresponding to the mature sequence of miR-126 (exogenous miRNA used as control); samples were then treated or not with Triton X-100 (1%) and incubated with or without RNase A (0.5 U) and T1 (15 U) for 30 min at 37 °C before RNA extraction. Mean ± S.E.M. of at least three independent experiments; * p < 0.05 vs. SHAM (panels A, B) or untreated (panel C).