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. 2019 Dec 30;21(1):251. doi: 10.3390/ijms21010251

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Two-photon microscopy analysis of CD31 expression in diaphyseal, metaphyseal and cortical bone vessels of twitcher mice. (A) 3D reconstruction of CD31⁺ vessels (in red) and stromal collagen expression revealed by second harmonic generation (in grey) in diaphysis and metaphysis of wt and twitcher mice (Z = 422 μm and 588 μm for wt and twi/twi specimens, respectively). (B) Cross-sectional view of CD31⁺ vessels in diaphysis and cortical bone (dashed white lines) of wt and twitcher mice (Z = 294 μm and 352 μm, respectively).