Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Psychosine affects the endothelial barrier integrity. HUVEC monolayer were treated overnight with 50 μM psychosine and immunostained with an anti-VE-Cad antibody (in green). Actin cytoskeleton was visualized by incubation with rhodamine phalloidin (in red) and nuclei were counterstained with DAPI (in blue). Note the loss of junctional protein organization and the appearance of endothelial gaps (asterisks) in the psychosine-treated HUVEC monolayer.
