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. 2020 Jan 5;21(1):348. doi: 10.3390/ijms21010348

Figure 5.

Figure 5

Prolonged oryzalin treatment increases mobility of AtFH13-YFP dots. (a) Epidermal cells co-expressing AtFH13-YFP and KMD-RFP mock-treated with water (control) and after 10 μM oryzalin treatment (+Oryz) for 10 min (no microtubule disruption observed) or 90 min (microtubules largely depolymerized). Single channel images are shown in grayscale, merged images display an overlay of the YFP channel in green with RFP in magenta. Particle tracks show trajectories of individual AtFH13-YFP dots over 50 s with insets magnified 2.5 times compared to the main image. (b) Box plot of mean velocities of AtFH13-YFP particles without, after 10 and after 90 min of oryzalin treatment. Scale bar is 10 µm. Two asterisks indicate a statistically significant difference (ANOVA, p < 0.001), NS: Non-significant. Construct abbreviations are defined in the text and in Figure 1.