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. 2019 Dec 31;21(1):271. doi: 10.3390/ijms21010271

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Physico-chemical characteristics of the Fe₃O₄NPs in mesenchymal stem cell neurogenic differentiation medium. (A) Size distribution obtained from dynamic light scattering measurements of Fe₃O₄NPs at concentrations of 10 and 25 μg/mL in mesenchymal stem cell neurogenic differentiation medium after 30 min, 24 and 48 h. (B) Physico-chemical properties of the Fe₃O₄NPs in mesenchymal stem cell neurogenic differentiation medium. (C) Phase-contrast micrographs of Fe₃O₄NPs in neurogenic medium at 10 and 25 μg/mL after 48 h: aggregations/agglomerations of the Fe₃O₄NPs were observed as brownish sediments, which increased as function of the concentration. Scale bar: 20 μm.