Figure 7.

Cytotoxic effects of Fe₃O₄NPs on hNLCs treated, at day 3 and 8 of transdifferentiation, for 24 and 48 h with increasing Fe₃O₄NPs concentrations (10–100 µg/mL). Evaluation by three cell viability assays. (A1,A2) Mitochondrial activity assessed by MTT assay; (B1,B2) cell viability evaluation by Trypan blue (TB) test; and (C1,C2) evaluation of intracellular ATP content. Data are expressed as percentage of viable cells (% of each control) and represent the mean ± S.D. * p< 0.05, statistical analysis by two-way ANOVA followed by Dunnett’s test.