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. 2019 Dec 25;21(1):155. doi: 10.3390/ijms21010155

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Increase of apoptosis in the cortico-medullary region of Atg7^flox/flox;KAP-Cre⁺ mouse kidney. (A) TUNEL staining in the cortico-medullary region of 2-month-old Atg7^flox/flox and Atg7^flox/flox;KAP-Cre⁺ kidney. Apoptosis in the cortico-medullary region of Atg7^flox/flox;KAP-Cre⁺ mouse kidney was detected using TUNEL staining. (B) Quantification of TUNEL positive cells of 2-month-old mice. TUNEL-positive signals were evaluated with ImageJ software (Atg7^flox/flox n = 4 and Atg7^flox/flox; KAP-Cre⁺ n = 5 *** p < 0.01). Statistical analyses were performed using a student’s t-test. Data in the graph are expressed as the mean ± SEM.