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. 2019 Dec 25;21(1):175. doi: 10.3390/ijms21010175

Figure 4.

Figure 4

Localization and expression of LC3 in the rat hippocampal neurons, after TMT treatment, in the CA4 area (#) are shown. Sections of the CA4 field at different intoxication time-points (3, 5, 7, and 14 days) are labeled for Neu N (green, a,d,g,j,m), LC3 (red, b,e,h,k,n), and Neu N/LC3 (merge, c,f,i,l,o). Control sections (a–c). At 3 days after treatment, marked LC3 immunoreactivity was observed (e) and LC3 labeling strongly increased at 5 days (h). LC3 staining decreased at 7 (k) and 14 days (n) post intoxication. Non-relevant LC3 reactivity in the Dentate Gyrus (*) was observed at any time of treatment (e,h,k,n). Note the progressive loss of Neu N-positive neurons in TMT-treated rats (d,g,j,m) compared with control rats (a). Arrows and inserts show details. Scale bar: 100 µm. Ctrl: control sample, d: days. For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.