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Impact of the G4 prone sequence on Δ40p53α transactivation activity from the PUMA RE. Δ40p53α was expressed under an inducible GAL1 promoter. Histograms show average fold induction over empty vector in three biological replicates (mean ± S.D.). The results obtained after 24 h in 2% galactose inducing media are presented. Asterisks indicate a significant induction of p53-dependent transactivation (p < 0.05).
