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. 2019 Dec 29;21(1):236. doi: 10.3390/ijms21010236

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of the profiles of ATP-induced degranulation between BMMCs and MCs/IL-9. (a) BMMCs (open circles) and MCs/IL-9 (closed circles) were stimulated with the indicated concentrations of ATP. The levels of degranulation were determined by measuring the enzymatic activity of β-hexosaminidase. Values are presented as the means ± SEM (n = 4). Multiple comparisons were performed using two-way ANOVA with the Sidak test. A value with ** p < 0.01 is regarded as significant. (b–e) The levels of mRNA expression of (b) P2rx1, (c) P2rx3, (d) P2rx4 and (e) P2rx7 in BMMCs (open circles) and MCs/IL-9 (closed circles) were measured by quantitative RT-PCR.