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. Author manuscript; available in PMC: 2020 Jan 25.
Published in final edited form as: Biomarkers. 2018 Jun 19;23(7):689–703. doi: 10.1080/1354750X.2018.1479771

Figure 7: qRT-PCR analysis of radiation responsive miRNAs in the blood samples of whole body irradiated non-human primates (NHP).

Figure 7:

NHP were whole body irradiated with a total dose of 4 Gy. Blood samples were drawn at D-8 (8 days prior to radiation), D1 (1-day post-radiation) and D2 (2 days post radiation). Total RNA was isolated from whole blood and 200 ng of total RNA was reverse transcribed using miScript RT II kit. A) qPCR was performed using miScript SYBRGreen and miScript primer assays for miR-17–5p, miR-106b-5p, miR-20a-5p. B) 200 ng of total RNA was reverse transcribed using Qiagen First Strand Synthesis Kit. qPCR assays were performed using Qiagen SYBR green master mix and qPCR primer assays against Rhesus monkey CDKN1A, THBS1, STAT5B, PTEN and JAK2.