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. 2019 Dec 20;21(1):59. doi: 10.3390/ijms21010059

Figure 6.

Figure 6

Co-cultivation of A549scr and A549shHsp70 cells with ThP1 cells led to tumor cell resistance to the anti-cancer drug etoposide. (A) A549scr and A549shHsp70 were co-cultivated with THP1 cells for 24 h. After THP1 cells were removed, 20 μM of etoposide was added. Micrographs of the carcinoma cells are shown. Scale bars 100 μm. (B,C) A549 cells with normal and downregulated Hsp70 were co-cultivated with THP1 cells and then were treated with etoposide (Eto). (B) Density plot of one representative experiment; (C) Percentage of apoptotic cells was measured with flow cytometry. Data from three independent experiments are summarized. ** p < 0.01. (D) Total amount of A549 cells co-cultivated with THP1 cells and survived after treatment with Eto was evaluated with cell counting using LUNA2 equipment. ** p < 0.01.