Table 1.
Percentage of neurons immunoreactive to individual neuroactive substance studied in the porcine duodenum in the control group (C group) and after low (LD group) and high (HD group) doses of acrylamide supplementation.
| MP | OSP | ISP | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Experimental Group | C Group | LD Group | HD Group | C Group | LD Group | HD Group | C Group | LD Group | HD Group |
| SP | 0.69 ± 0.14 | 1.05 ± 0.25 (**) | 2.67 ± 0.44 (**) | 21.47 ± 1.19 | 25.83 ± 1.28 (***) | 38.50 ± 1.23 (***) | 11.57± 1.17 | 23.51± 1.18 (**) | 26.77± 1.75 (***) |
| CGRP | 12.38 ± 1.02 | 21.75 ± 0.90 (***) | 31.54 ±0.70 (***) | 14.62 ± 1.20 | 16.15 ± 0.68 | 27.44 ± 0.99 (***) | 20.14 ± 0.69 | 22.59 ± 0.65 (*) | 28.64 ± 0.77 (***) |
| GAL | 2.87 ± 0.41 | 6.45 ± 0.70 (***) | 10.24 ± 0.32 (***) | 31.75 ± 1.41 | 39.10 ± 0.81 (***) | 49.78 ± 0.64 (***) | 38.86 ± 1.53 | 49.12 ± 1.15 (***) | 54.45± 1.35 (***) |
| nNOS | 29.34 ± 1.78 | 31.55 ± 1.12 | 37.39 ± 0.98 (**) | 2.41 ± 0.60 | 4.62 ± 0.28 (*) | 9.20 ± 0.46 (***) | 1.53 ± 0.19 | 2.84 ± 0.26 | 7.46 ± 0.65 (***) |
| VACHT | 13.92 ± 0.91 | 20.22 ± 0.46 (**) | 24.89 ± 1.50 (***) | 20.80 ± 1.00 | 26.88 ± 0.69 (**) | 33.60 ± 1.48 (***) | 42.74 ± 1.75 | 52.81 ± 1.43 (**) | 61.96 ± 1.06 (***) |
MP—myenteric plexus, OSP—outer submucous plexus, ISP—inner submucous plexus, SP—substance P, CGRP—calcitonin gene-related peptide, GAL—galanin, nNOS—neuronal nitric oxide synthase, VACHT—vesicular acetylcholine transporter. * p < 0.05, ** p < 0.01, *** p < 0.001.