Figure 2.

Schematic representation of TLQP-21 intracellular transduction mechanism in Chinese Hamster Ovary (CHO-K1) cells. TLQP-21, by binding a G protein coupled receptor (GPCR), activates Phospholipase C (PLC)-β that in turn produces Diacylglycerol (DAG) and Inositol Trisphosphate (IP3) as second messengers. These molecules activate Protein kinase C (PKC), stimulate extracellular signal-regulated kinase (ERK)1/2 phosphorylation and induce intracellular Ca²⁺ release from the endoplasmic reticulum (ER) with the subsequent Ca²⁺ entry from outside the cell, mediated by the stromal interaction molecules (STIM) and calcium release-activated calcium channel (CRAC)-Orai1 interaction. Phosphorylation of AKT is probably a result of the increase in cellular Ca²⁺ concentrations.