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. 2019 Dec 20;21(1):57. doi: 10.3390/ijms21010057

Figure 1.

Figure 1

GR mRNA expression in sex-specific FDLE cells. FDLE cells were isolated from fetal rats. Data bars represent the relative fold change (RFC) + SEM. (A) FDLE cells were cultured in SS-Med or SF-Med. GR mRNA expression was significantly higher in females cultured in SS-Med compared to equally treated male FDLE cells (n = 8, * p < 0.05, T-test). No significant sex difference was observed for GR mRNA expression in SF-Med. (B) RNA directly isolated from FDLE cells without prior cell culture showed significantly higher GR mRNA expression in females (n = 6, ** p < 0.01, T-test). (C) Testosterone (T) or dihydrotestosterone (DHT), in SF-Med, had no effect on GR mRNA expression in male and female cells (n = 8). (D) Progesterone (P) and estradiol (E2), in SF-Med, did not alter GR mRNA expression of male and female FDLE cells (n = 6). (□) male, (■) female.