Figure 1.

Experimental approaches for the study of hypoxia in neuroblastoma. Connecting molecular characterisation with information directly obtained from in vivo assays in hypoxia chambers and/or in vitro cultured spheroids would allow the generation of relevant evidence-driven hypothesis. 3D systems can be used for ex vivo or in vitro testing. Decreased oxygen concentration is symbolized in the illustration as red arrow pointing down or color gradient blue to red. Green arrows denote possible workflows.