Figure 3.

Inhibition of FGF-signaling has no impact on the processing of double-strand breaks (DSBs) and RPA loading. GIST T-1R cells were labeled with 10 µg/mL BrdU for 4 h prior to DNA damage (0.25 µg/mL doxorubicin for 4 h) following washout for 24 h in absence and presence of BGJ398 (1 µM), a selective FGFR1-4 inhibitor. Immunofluorescent detection of incorporated BrdU without denaturation and phosphor-RPA was performed. DAPI staining was used to outline the nucleus. (Scale bars = 10 μm).