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. 2020 Jan 5;21(1):352. doi: 10.3390/ijms21010352

Figure 5.

Figure 5

FGFR2 knockdown sensitizes GISTs to topoisomerase II inhibitors and induces their apoptosis. (A) GIST T-1R cells were transfected with scrambled siRNA (control) or siRNA targeting FGFR2 for 48 h. The expression of FGFR-1,2 was analyzed by western blotting. Actin was used as a loading control. (B) The light microscopy images of doxorubicin (Dox)-treated GIST cells (0.5 µg/mL for 48 h) previously transfected with scrambled siRNA (control) or siFGFR2 for 48 h. (C) Representative flow cytometry histograms showing the cell-cycle distribution of GIST T-1R cells transfected with control siRNA or siRNA FGFR2 for 48 h and treated with DMSO or Dox (0.5 µg/mL) for another 48 h. Experiments were conducted in quadruplicate and at least 150,000 cells were counted per experiment. The increase of apoptotic cells was evidenced by a sub-G1 peak in cells depleted of FGFR2 and treated with Dox (noted by arrow). (D) Quantification of apoptotic cells was measured by flow cytometry, * p < 0.05.