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. 2020 Jan 24;17:34. doi: 10.1186/s12974-020-1711-1

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Hypomethylation of CpG islands of the NGF gene promoter after CFA treatment. a Online software prediction of CpG islands around the NGF gene promoter region. The light blue areas on the map indicate the potential CpG islands. b The representative MSP assay showed DNA methylation and unmethylation ratio of the CpG islands in the NGF gene promoter region of L4–6 DRGs was significantly decreased after 3 days of CFA treatment (*P < 0.05, compared with the control group, n = 4). c Schematic of CpG island showing locations of the 12 CpG sites in the NGF gene promoter area d BSP sequencing, CFA injection resulted in a significant percentage of methylated CpG sites decrease to 16.7% in CpG islands of the NGF gene promoter region. e, f The expression of DNMT3b mRNA and protein in DRGs were significantly decreased after CFA treatment (3 days after CFA injection) (*P < 0.05, compared with the control group, n = 4), while DNMT3a level did not change significantly