Fig. 4.

DNMT3b is a direct target of miR-29b in DRGs. a The expression of miR-29b was significantly increased in DRGs after CFA treatment (*P < 0.05, compared with the control group, n = 4). b The relative expression level of miR-29b when transfected with its inhibitor and mimic compared with scramble or miR-CON group, respectively (*P < 0.05, #P < 0.05). c,d Western blot analysis showed that overexpression of miR-29b inhibited the protein level of DNMT3b, and inhibition of miR-29b expression promoted the expression of DNMT3b protein (*P < 0.05, #P < 0.05). e The reverse binding sequence of mature miR-29b inserted into C-terminal of the luciferase gene to generate pGL3-DNMT3b -3’UTR. f miR-29b targeted the wild type but not the mutant 3’UTR of DNMT3b (*P < 0.05)