Table 1.
Primers and parameters of RT-qPCR
| RNA | Sequences forward/backward 5′-3′ | Temperature (°C), step 1/2/3 | Duration (s), step 1/2/3 |
|---|---|---|---|
| iNOS |
GCTTGTCTCTGGGTCCTCTG/ CTCACTGGGACAGCACAGA |
95/59/72 | 15/15/45 |
| CD206 |
AACAAGAATGGTGGGCAGTC/ CCTTTCAGTCCTTTGCAAGC |
95/56/72 | 15/15/45 |
| TNFα |
CATCCGTTCTCTACCCAGCC/ AATTCTGAGCCCGGAGTTGG |
95/56.6/72 | 15/15/45 |
| IL-6 |
CCCAACTTCCAATGCTCTCCT/ AGCACACTAGGTTTGCCGAG |
95/57.3/72 | 15/15/45 |
| IL1-β |
GACTTCACCATGGAACCCGT/ GGAGACTGCCCATTCTCGAC |
95/56/72 | 15/15/45 |
| IL-10 |
GAAAAATTGAACCACCCGGCA/ TTTCCAAGGAGTTGCTCCCG |
95/56/72 | 15/15/45 |
| RPL13a | TCTCCGAAAGCGGATGAACAC/ CAACACCTTGAGGCGTTCCA | 15/15/45 |