Figure 4.

Interaction between Nr4a1 and LKB1 activates AMPK/SIRT1 signaling pathway and inhibits activation of NF-κB signaling pathway. (A) Relative mRNA expression of AMPK, SIRT1 and NF-κB in T2DM rats measured by RT-qPCR; (B) Protein levels and phosphorylation levels of AMPK, ACC, SIRT1, IκBα and p-p65 measured by western blot analysis. Repetition=3. n=3, Data were analyzed by t test. * For pair-wise comparison, p<0.05. Nr4a1 – nuclear receptor subfamily 4 group A member 1; LKB1 – liver kinase B1; AMPK – adenosine monophosphate-activated protein kinase; ACC – acetyl-CoA carboxylase; SIRT1 – sirtuin-1; T2DM – type 2 diabetes mellitus; NF-κB – nuclear factor-kappa B; RT-qPCR – reverse transcription quantitative polymerase chain reaction.