Table 2.
Antibodies used in Western blot analysis.
| Antibody | Item No. | Dilution ratio |
|---|---|---|
| Nr4a1 | ab13851 | 1/500 |
| LKB1 | ab15095 | 1/100 |
| p-AMPK | ab23875 | 1/1000 |
| AMPK | ab32047 | 1/1000 |
| p-ACC | ab68191 | 1/5000 |
| ACC | ab72046 | 1/2000 |
| SIRT1 | ab110304 | 0.125 μg/mL |
| p-p65 | ab86299 | 1/2000 |
| p65 | ab16502 | 0.5 μg/mL |
| IκBα | ab32518 | 1/1000 |
| p-IκBα | ab133462 | 1/10000 |
| β-actin | ab179467 | 1/5000 |
Nr4a1 – nuclear receptor subfamily 4 group A member 1; LKB1 – liver kinase B1; AMPK – adenosine monophosphate-activated protein kinase; ACC – acetyl-CoA carboxylase; SIRT1 – sirtuin-1.