(a) Concordance amongst somatic alterations (sSNVs, indels
and CNAs) in known CRC ‘driver’ genes between paired primary CRCs
and metastases. Stacked barplots illustrate the total number of sSNVs and indels
in exonic regions with a lower cutoff of variant allele frequency (VAF)=0.1 in
the corresponding site (primary or metastasis). (b) The percentage
of clonal sSNVs that are shared, primary-private, or
metastasis-private out of all clonal sSNVs with CCF>60%
in any of paired primaries and distant metastases. (c) Violin plots
illustrate the probability density of driver gene fold enrichment amongst
shared, primary-private, and metastasis-private clonal
non-silent sSNVs based on known CRC or pan-cancer ‘drivers’. The
inset box corresponds to the 25th to 75th percentile (interquartile range, IQR);
the horizontal line indicates the median; and the vertical line includes data
within 1.5 times the IQR. A test statistic was computed based on n=100
down-samplings amongst patients (Methods).
P-value, Wilcoxon Rank-Sum Test (two-sided).