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. Author manuscript; available in PMC: 2020 Jun 23.
Published in final edited form as: Nat Chem Biol. 2019 Dec 23;16(2):122–125. doi: 10.1038/s41589-019-0433-0

Fig. 1.

Fig. 1.

The exposed phospho-tyrosine recognition loop of PTP1B-OX interacts with 14-3-3ζ. (a) Close-up view of the pTyr recognition loop of reduced PTP1B (PDB code: 2HNQ) and (b) of PTP1B-OX (sulphenyl-amide species, PDB code 1OEM). (c) Reversible oxidation of PTP1B in cells treated with EGF (100 ng/ml) for the indicated times. This experiment was repeated three independent times with representative data shown. (d) Effect of scavenging ROS on the PTP1B–14-3-3ζ association in cells incubated with the antioxidant NAC (1 mM, 1 h) and stimulated with EGF for the indicated times. This experiment was repeated two independent times with representative data shown. Uncropped images are shown in Supplementary Fig. 12.