Table 2.
Precision, accuracy, extraction recovery and matrix effect of butylidenephthalide, ligustilide, senkyunolide A and ferulic acid in rat plasma. (n = 3 days and six replicates per day).
| Component | Added (ng/mL) |
Precision (RSD%) | Accuracy (RE%) | Extraction Recovery | Matrix Effect | |||
|---|---|---|---|---|---|---|---|---|
| Intraday | Interday | Mean (%) | RSD (%) | Mean (%) | RSD (%) | |||
| Butylidene-phthalide | 7.08 | 8.49 | 10.97 | 4.77 | 106.38 | 2.22 | 93.82 | 9.06 |
| 141.50 | 11.05 | 12.71 | 11.70 | 103.41 | 9.33 | 100.88 | 6.38 | |
| 1132.00 | 12.88 | 10.07 | 4.83 | 105.70 | 3.57 | 95.63 | 4.68 | |
| Ligustilide | 5.22 | 6.94 | 11.39 | 1.87 | 92.61 | 3.68 | 98.13 | 5.00 |
| 104.40 | 11.04 | 10.25 | 7.85 | 94.71 | 3.05 | 100.01 | 3.39 | |
| 835.20 | 9.49 | 11.44 | −2.75 | 97.58 | 2.80 | 93.07 | 2.43 | |
| Senkyuno-lide A | 6.55 | 5.17 | 7.40 | 6.54 | 101.54 | 2.76 | 92.29 | 3.38 |
| 131.00 | 11.16 | 10.92 | 8.05 | 110.78 | 6.32 | 92.00 | 2.41 | |
| 1048.00 | 10.84 | 9.67 | 0.82 | 98.14 | 2.43 | 98.47 | 1.28 | |
| Ferulic acid | 6.68 | 5.70 | 8.06 | −8.14 | 109.05 | 12.39 | 105.63 | 1.34 |
| 667.50 | 8.55 | 6.43 | 9.00 | 101.69 | 6.69 | 95.48 | 2.57 | |
| 10680.00 | 6.18 | 9.36 | 1.26 | 107.63 | 5.81 | 91.23 | 3.42 | |