Skip to main content
. 2019 Dec 21;25(1):46. doi: 10.3390/molecules25010046

Figure 5.

Figure 5

Impact of P6 peptide, colchicine, and colchicine-P6 peptide conjugate treatments on monosodium urate (MSU) crystal phagocytosis by human THP-1 macrophages. THP-1 macrophages were pre-treated with the P6 peptide, colchicine, or colchicine-P6 peptide (1, 10, or 25 μM) for 24 h followed by treatment with MSU crystals (100 μg/mL) for 4 h. Data are presented as scatter plots of three independent experiments with the mean and standard deviation highlighted. * p < 0.001. (A) Representative flow cytometry plots depicting increased macrophage cell population side-scatter in MSU-treated THP-1 macrophages, indicative of MSU phagocytosis. Treatments with P6 peptide, colchicine, or P6 peptide-colchicine (10 μM) appeared to reduce macrophage cell clustering in the P2 region indicative of reduced MSU phagocytosis. (B) P6 peptide (10 and 25 μM), colchicine (1, 10, and 25 μM) and P6 peptide-colchicine (1, 10, and 25 μM) treatments reduced MSU phagocytosis by THP-1 macrophages.