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. 2020 Jan 2;25(1):186. doi: 10.3390/molecules25010186

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of pterostilbene (PST) supplementation on muscular angiogenesis in exercise training rats. Paraffin sections were obtained from soleus muscles and stained for CD31 (a marker of endothelial cells) (200 × objective) (A). Capillary to fiber ratio (B) and vascular density (C) were determined by counting the CD31+ vascular structures from three independent fields of view per animal. Genes expressions (D), representative western blotting bands (E) and their quantitative analysis (F) of VEGF from soleus. Scale bar, 100 μm. Protein expression levels were normalized to the expression of β-actin. Data are expressed as mean ± SD, a—p < 0.05 compared with Sed group; b—p < 0.05 compared with Ex group.

Effects of pterostilbene (PST) supplementation on muscular angiogenesis in exercise training rats. Paraffin sections were obtained from soleus muscles and stained for CD31 (a marker of endothelial cells) (200 × objective) (A). Capillary to fiber ratio (B) and vascular density (C) were determined by counting the CD31+ vascular structures from three independent fields of view per animal. Genes expressions (D), representative western blotting bands (E) and their quantitative analysis (F) of VEGF from soleus. Scale bar, 100 μm. Protein expression levels were normalized to the expression of β-actin. Data are expressed as mean ± SD, a—p < 0.05 compared with Sed group; b—p < 0.05 compared with Ex group.