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. 2020 Jan 1;25(1):181. doi: 10.3390/molecules25010181

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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High-performance liquid chromatography (HPLC) traces (254 nm) of IFA standards after transglycosylation using DGAS. (a) Isoflavone standard mixture, (b) transglycosylated glycitein, (c) transglycosylated daidzein, and (d) transglycosylated genistein. Peaks: GD, daidzin; GL, glycitin; GG, genistin; MD, malonyldaidzin; ML, malonylglycitin; AD, acetyldaidzin; AL, acetylglycitin; MG, malonylgenistin; DN, daidzein; LN, glycitein; AG, acetylgenistin; GN, genistein. Peaks labeled LN₁ to LN₆, DN₁ to DN₈, and GN₁ to GN₆ are transglycosylated products from each IFA. Enzymatic transglycosylation reactions were performed using 1.0 mM of glycitein, 5.0 mM of daidzein, or 5.0 mM of genistein with 5.0 U of DGAS and 2.0 M of sucrose.