Table 1.
Conversion yields of soy isoflavone aglycones after Deinococcus geothermalis amylosucrase (DGAS)-treated transglycosylation according to concentrations of donor and acceptors and amounts of the DGAS enzyme.
| DGAS (Units) |
Sucrose (M) |
Glycitein (mM) | Daidzein (mM) | Genistein (mM) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0.05 | 1.0 | 4.0 | 0.2 | 5.0 | 20 | 0.2 | 5.0 | 20 | ||
| 0.5 | 0.1 | 0.0 ± 0.0 c 1 | 1.9 ± 1.7 c | 3.9 ± 0.2 b | 0.0 ± 0.0 f | 4.3 ± 0.6 de | 2.4 ± 0.4 ef | 10.9 ± 2.3 c | 3.1 ± 1.3 de | 2.1 ± 0.1 e |
| 1.0 | 0.0 ± 0.0 c | 6.8 ± 0.8 a | 4.2 ± 0.2 b | 20.0 ± 2.5 b | 8.3 ± 1.0 cd | 2.7 ± 0.1 ef | 59.8 ± 0.4 b | 5.3 ± 0.3 d | 0.8 ± 0.0 e | |
| 2.0 | 0.0 ± 0.0 c | 6.5 ± 0.8 a | 4.9 ± 0.4 ab | 28.8 ± 3.0 a | 9.2 ± 1.0 c | 1.8 ± 0.2 ef | 68.6 ± 1.0 a | 3.5 ± 0.1 de | 1.2 ± 0.0 e | |
| 1.0 | 0.1 | 0.0 ± 0.0 d | 7.0 ± 0.2 c | 6.0 ± 0.3 c | 22.5 ± 3.2 d | 12.0 ± 0.9 e | 4.4 ± 1.1 f | 19.3 ± 1.9 d | 7.1 ± 0.6 e | 1.3 ± 0.0 e |
| 1.0 | 13.3 ± 2.5 b | 19.0 ± 1.7 a | 8.8 ± 0.4 bc | 50.0 ± 2.4 b | 32.5 ± 0.7 c | 6.9 ± 0.1 f | 53.1 ± 4.2 b | 28.5 ± 0.2 c | 4.8 ± 0.0 e | |
| 2.0 | 8.6 ± 3.6 bc | 20.8 ± 0.4 a | 23.2 ± 3.7 a | 55.5 ± 2.2 a | 32.4 ± 0.8 c | 4.8 ± 0.2 f | 62.5 ± 4.7 a | 24.7 ± 0.2 cd | 2.9 ± 0.2 e | |
| 5.0 | 0.1 | 20.8 ± 6.4 e | 35.0 ± 1.6 d | 28.2 ± 0.8 de | 72.0 ± 1.5 b | 65.0 ± 0.8 c | 23.3 ± 0.3 f | 77.3 ± 2.7 b | 74.8 ± 0.5 bc | 23.4 ± 0.0 e |
| 1.0 | 16.3 ± 1.5 e | 69.5 ± 9.2 b | 56.8 ± 2.2 c | 97.2 ± 2.8 a | 95.0 ± 0.1 a | 50.5 ± 0.0 d | 92.2 ± 5.1 a | 96.6 ± 1.0 a | 66.0 ± 0.2 d | |
| 2.0 | 18.0 ± 2.8 e | 88.8 ± 3.8 a | 52.1 ± 1.3 c | 97.5 ± 0.7 a | 96.9 ± 0.2 a | 36.9 ± 0.1 f | 92.1 ± 3.2 a | 98.2 ± 0.1 a | 70.1 ± 0.1 cd | |
1 Data are expressed as means ± standard deviations (n = 3). Means with different superscripted letters indicate that for the same amount of DGAS, each isoflavone aglycone differed significantly by the Tukey–Kramer honestly significant difference test (p < 0.05).