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. 2019 Dec 22;25(1):52. doi: 10.3390/molecules25010052

Figure 1.

Figure 1

Schematic of the protospacer adjacent motif (PAM)-based guide RNA design for the targeting of the 2573T > G endothelial growth factor receptor (EGFR) point mutation. (A) Shown are the sequence of wild type EGFR (2573T), in grey, and the ribonucleoprotein complex comprised of CRISPR-Cas9 and guide RNA, in cyan. The PAM sequence is shown in red characters. (B) Schematic of the mutant sequence of EGFR (2573T > G), CRISPR-Cas9, and guide RNA. Lower-case letters indicate the 2573T > G mutation of EGFR. (C) Design of the four sgRNAs with single-base mismatches in PAM or within the guide RNA complementary sequences, indicated in lower-case letters.