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Schematic of the targeting of the RFP-GFP reporter system by CRISPR-Cas9. (A,B) Shown are the reporter construct with the wild type EGFR (2573T) and mutant EGFR (2573T > G) sequence flanked by red fluorescent protein (RFP) and green fluorescent protein (GFP). The PAM-based sgRNA sequence and its PAM are shown in blue and red, respectively.
