Figure 3.

Comparison of the specificities of the PAM-based and sequence-complementarity methods for genome editing of targets with single-base differences. (A) Flow cytometry analyses of the genome editing specificities of the sgRNAs targeting the RFP-GFP dual reporters; (B) the graphs represent the ratio of the GFP population divided by whole population (RFP+GFP) in the WT and MT reporter systems, respectively. (C) The relative specificities are calculated by dividing the ratio of the green fluorescence protein (GFP) population in the MT reporter by that of the WT reporter. Lower-case letters indicate the mutated sequence (2573T > G). Red letters indicate the PAM sequences.