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. 2019 Dec 22;25(1):52. doi: 10.3390/molecules25010052

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Indel patterns and off-target analyses by T7E1 assay. (A) Shown are the most abundant 10 indel patterns of the on-target site by genome editing with sgRNA-1. The PAM sequence and insertions are indicated by red and blue characters, respectively. (B) Shown are the sequences of potential 15 off-target sites. Mismatches to the on-target sequence and PAM sequences are indicated by lower-case and red characters, respectively. (C) Shown are the indel rates of the on-target and the 15 candidate off-target sites that were quantified by targeted deep sequencing.