Table 1.
DNA Primer Sequence Information for deep sequencing assay of off-target sites. Mis-matched nucleotides are shown in lower-case letters.
| Target Gene (Primer Direction) |
Primer Sequence (5′ to 3′) | Off-Target Sequence |
|---|---|---|
| EGFR_Off_1_F1 | TAAATTGAGTGCAGAGCCTTG | TCAAGATCACAcATaTTGGGAGG |
| EGFR_Off_1_R1 | GCAATGGTACCCTTTTCTTC | |
| EGFR_Off_1_F2 | AAACCCTGTGTTTGGAATTCA | |
| EGFR_Off_1_R2 | TTTGTTGTCCTTATATGTCATTGTG | |
| EGFR_Off_2_F1 | TCAGAGCCCAGTTTATCATAG | TCAAtATgACAtATTTTGGGGGG |
| EGFR_Off_2_R1 | GTAAATTAGCTGGTCTCAGG | |
| EGFR_Off_2_F2 | GGCATCAAGTAAATTACTCAAAACA | |
| EGFR_Off_2_R2 | CATGTAGCCAAAACAAAACAAACA | |
| EGFR_Off_3_F1 | ATTCAACAACGATCTGTTGTG | TgAAGgTCACAGAgTTTGGGAGG |
| EGFR_Off_3_R1 | AAAGCAAAACCCCAAAGCGA | |
| EGFR_Off_3_F2 | TCTAGTGGAGACAGATGTTAAAC | |
| EGFR_Off_3_R2 | GGGCTTTCCTCTCTTCTGT | |
| EGFR_Off_4_F1 | TGCATTTTGACTCTTCTCGA | TgAAGAcCACAGATTTaGGGAGG |
| EGFR_Off_4_R1 | AGAAGAAAGCTTCTGATGCC | |
| EGFR_Off_4_F2 | ATATCCAATAACAACAATACTAGC | |
| EGFR_Off_4_R2 | CCATCATTGAATTGAGGTCAC | |
| EGFR_Off_5_F1 | GGAAATCAAATTTGGGGTTG | TCAAGATgACAGATTTgGGtGGG |
| EGFR_Off_5_R1 | CTGCTGTTCCCCAACTTATT | |
| EGFR_Off_5_F2 | TCTGAGACTGGGTCATTCAT | |
| EGFR_Off_5_R2 | GCTTTTTACAACTATCTTACTAATAAC | |
| EGFR_Off_6_F1 | CCTTGTTGATTTACATTGATGTG | TCAAGATCACAaATaTTGGtAGG |
| EGFR_Off_6_R1 | CATTCCATAACATTCTCAGGT | |
| EGFR_Off_6_F2 | CCAATGTAAATCAACAAGGGT | |
| EGFR_Off_6_R2 | AATCATGAACATATTTATGCTTTCC | |
| EGFR_Off_7_F1 | CAAACTCACAATTGTGAGGG | TCAAcATaAtAGATTTTGGGAGG |
| EGFR_Off_7_R1 | AGCAGAGCATGGAGCTCATA | |
| EGFR_Off_7_F2 | ATGGACCGGGTGGCTTAAA | |
| EGFR_Off_7_R2 | AATGAAGCATGGTGACAGAC | |
| EGFR_Off_8_F1 | ACCAGGATGGTCTTGATCTC | aCAAGgTCAtAGATTTTGGGAGG |
| EGFR_Off_8_R1 | AAGTGTTTAAAACAGTGCCA | |
| EGFR_Off_8_F2 | CTGCCCAGGCTTCATCTTAA | |
| EGFR_Off_8_R2 | CCAGGCTGAAATGATCAAAG | |
| EGFR_Off_9_F1 | ATTTCAAAGGGTGGGGCTTT | aCAAGAaCACAGATTTTGGcAGG |
| EGFR_Off_9_R1 | GAAGTCTCAGATCAAGGTCC | |
| EGFR_Off_9_F2 | TTGGGACTTGTCATCCTTTT | |
| EGFR_Off_9_R2 | GGAGAAGAGCATGAGTGCTA | |
| EGFR_Off_10_F1 | TAGACTAGTCACCAGAATTCC | TCAAGATCAaAaAcTTTGGGAGG |
| EGFR_Off_10_R1 | TCACCATGCAGTTGTACATA | |
| EGFR_Off_10_F2 | CAAGGAAGACAAAAGAGACA | |
| EGFR_Off_10_R2 | TCCCCAGTCTGTTCTCTCTT | |
| EGFR_Off_11_F1 | GTTATGTGAGGTGTTTGTGT | TCAAGATCACAGATTTTtGGTGG |
| EGFR_Off_11_R1 | AGAACACACCATGTTAGAGG | |
| EGFR_Off_11_F2 | TGATGATAATTTCATGTGTGTTACC | |
| EGFR_Off_11_R2 | ACTCTCTTTGGTGAGAAGGA | |
| EGFR_Off_12_F1 | TACAGTTGTGTGGCTTTCGA | TaAAGtTCACAGgTTTTGGGGGG |
| EGFR_Off_12_R1 | GCCGTCTCAATACTTGTGAA | |
| EGFR_Off_12_F2 | AACAACTATGGTATGGGCCA | |
| EGFR_Off_12_R2 | GGTAAAACCCCATCTACTAAAAA | |
| EGFR_Off_13_F1 | GCCTCTTATCTGAACGAGAA | TCAAGgcCACAcATTTTGGGTGG |
| EGFR_Off_13_R1 | ATTAGTTGCAGTTCAAAGCC | |
| EGFR_Off_13_F2 | AAATCTACTTGGAGCAATGC | |
| EGFR_Off_13_R2 | TTTTTGGTAGGAGCCTGCAG | |
| EGFR_Off_14_F1 | AGTGTTTCGATAGATGGAGG | TgAAGAaCACAGtTTTTGGGTGG |
| EGFR_Off_14_R1 | CTACCCAAAACCTTTGTCCC | |
| EGFR_Off_14_F2 | AGAAGAGCACGAGTGGTAAA | |
| EGFR_Off_14_R2 | CACCCTTTTTCTTCCTCCAT | |
| EGFR_Off_15_F1 | GGTGGGGAAAAAAGTTTTTGG | TgAAGAaCACAGtTTTTGGGTGG |
| EGFR_Off_15_R1 | CTACACCTTCTTTTCCCGAC | |
| EGFR_Off_15_F2 | GAGTGACGAGGAGGAGGAAA | |
| EGFR_Off_15_R2 | TCAACACCCTTTTCCCCAT | |
| EGFR_on-target_F | TACTTGAAGCTGTCCTTCCC | TCAAGATCACAGATTTTGGG |
| EGFR_on-target_R | CCGTCGTCCTTGAAGAAGAT |