Figure 2.

Effect of ferrugone and DMI on apoptotic cell death in human ovarian cancer cells. A2780 cells were treated with ferrugone (A) and DMI (B) (0.25, 0.5, and 1 µM) for 48 h and co-stained with PI and Annexin V-FITC (fluorescein isothiocyanate). The translocation of phosphatidyl serine was detected by flow cytometry. The graph indicates the percentages of annexin V-positive apoptotic cells in the right quadrants of flow cytometry graphs. The data are representative of three independent experiments. Data were analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test. * p < 0.05 as compared with untreated group.