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. 2020 Jan 3;25(1):207. doi: 10.3390/molecules25010207

Figure 3.

Figure 3

Involvement of caspases in ferrugone and DMI-induced cell death in human ovarian cancer cells. A2780 cells were pretreated with broad caspase inhibitor z-VAD-fmk (50 µM) for 2 h, and then treated with ferrugone (A) and DMI (B) (0.5 µM) for 48 h. Annexin/PI V-FITC staining assay was performed to determine apoptosis. The graph indicates the percentages of annexin V-positive apoptotic cells in the right quadrants of flow cytometry graphs. The data are representative of three independent experiments. Student’s t-test (two-tailed) was applied to evaluate the significance. # p < 0.05 as compared with the untreated group. * p < 0.05 as compared with the ferrugone or DMI only-treated group.