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. 2019 Dec 18;25(1):13. doi: 10.3390/molecules25010013

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Structure of myrtucommuacetalone 1 (MCA-1). (B) Effect of myrtucommuacetalone 1 (MCA-1) on nitric oxide (NO) release by activated THP1 monocytes. THP Cells (2 × 10⁵) were incubated in the presence (25, 5, 0.5, 0.25 µg/mL) or absence of the compound (control) for 48 h, and (C) the effects of the compound at 25 µg/mL on the basal level of neuronal NO in U 138MG neuronal cells were observed. The supernatant was analyzed for the presence of nitrite using the Griess method. Values are expressed as mean ± SD for triplicate experiments. The results were compared with N^G Monomethyl L arginine (LNMA), a known inhibitor of NO. * p < 0.05, ** p < 0.005.

(A) Structure of myrtucommuacetalone 1 (MCA-1). (B) Effect of myrtucommuacetalone 1 (MCA-1) on nitric oxide (NO) release by activated THP1 monocytes. THP Cells (2 × 10⁵) were incubated in the presence (25, 5, 0.5, 0.25 µg/mL) or absence of the compound (control) for 48 h, and (C) the effects of the compound at 25 µg/mL on the basal level of neuronal NO in U 138MG neuronal cells were observed. The supernatant was analyzed for the presence of nitrite using the Griess method. Values are expressed as mean ± SD for triplicate experiments. The results were compared with N^G Monomethyl L arginine (LNMA), a known inhibitor of NO. * p < 0.05, ** p < 0.005.